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Concentration (start) × Volume (start) = Concentration (final) × Volume (final) Protocols Multiplex immunofluorescence IHC Multiplex immunofluorescence IHC detects multiple protein biomarkers in one tissue section by sequential antibody staining, HRP-mediated tyramide fluorophore deposition, heat-mediated antibody stripping, nuclear counterstaining, multispectral imaging, spectral unmixing, and digital cell phenotyping
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