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MRD cells were also generated in vivo, by treating mouse xenografts of the 105K Tsc2 β/β RCC line in syngeneic C57BL/6J mice with rapamycin (3 mg/kg, intraperitoneally [IP] 3 times/wk for 1 month, MRD525), or the bi-steric inhibitor RMC-6272 (8 mg/kg, IP once/wk for 1 month, MRD639), followed by 2 months of treatment cessation (Figure 1A)
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Examples include longitudinal investigations of telomerase-associated pathways, telomere-maintenance mechanisms, transcription-associated signaling processes, circadian regulatory systems, and other studies involving multiple sampling timepoints
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