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Bacterial cells were resuspended in buffer A (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 5% glycerol), disrupted by sonication, and then centrifuged at 27,000 g for 20 min at 4 °C
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