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utilizing a batch treatment, such as by incorporating the initial mixture to the solid phase in a vessel, blending, separating the solid phase, and getting rid of the washing in the liquid phase, centrifuging again, and adding the elution buffer, re-centrifuging and removing the elute.Sometimes a hybrid method is employed so that the batch method is used for binding, however, the solid phase containing the bound target molecule is packed onto a column and washing and elution are carried out on the column

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Therefore, we have seen an important role of APXs in protecting plants against heavy metal stress in soil from different scientific reports