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Briefly, a stromal vascular fraction was produced with a collagenase enzymatic method, and then, cells were plated in 25 cm 2 cell culture flasks at a density of 2 × 10 5 living cells/cm 2 and cultured in low-glucose Dulbecco’s Modified Eagle Medium (Biosera, Nuaille, France), supplemented with 10% fetal calf serum (Biosera, Nuaille, France) and 1% Antibiotic–Antimycotic Solution

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Why it was included in the FDA ban The FDA cites “risk for immunogenicity, peptide-related impurities, and limited safety-related information” as reasons for the BPC-157 ban

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