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While freezing did not impact the concentrations of NAD + , NADH, GSH, or GSSG, it triggered the conversion of NADPH to NADP+, with about 50±8% of NADP + originating from oxidized NADPH (Supplementary Figure 1m-r)

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The engineering of a CxxC-type Grx with a CGFS-type loop switched its function from oxidoreductase to FeS transferase in a zebrafish model and the introduction of a CxxC-type loop into a CGFS-type Grx abolished its FeS transferase activity and activated the oxidative half-reaction (Figure 2, reaction 5 reverse) of the oxidoreductase [92]