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GST pull-downs Rosetta ™ Competent Cells (Novagen) transformed with Pgex-GST-Ran(WT), Pgex-GST-Ran(Q69L) and Pgex-GST-Ran(T24N), Pgex-GST-PlexinB1(cyto), Pgex-GST-PlexinB2(cyto), Pgex-GST-PlexinB3(cyto) or RanBP1(RBD)-GST were grown overnight in Luria Broth and glutathione-S-transferase (GST) fusion protein expression induced with IPTG 1 mM for 3 h
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