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The following antibodies were used for immunofluorescence: anti-4HNE (1:200, Abcam, ab46545), anti-GPX4 (1:400, Abcam, ab125066) and anti-GR-1 (1:100, BioLegend, 108413) as primary antibodies and anti-rabbit IgG Alexa Fluor 488 (1:1000, Invitrogen, A-11034) and anti-rat IgG Alexa Fluor 594 (1:1000, Invitrogen, A-11007) as secondary antibody
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Reflex cutaneous vasodilation was found to be appreciably attenuated in all skin grafts (753), with the effect persisting for more than 4 years postsurgery and likely for the life of the burn survivor (FIGURE 11) (754)
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