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Neutrophils suspended in HBSS containing CaCl 2 , MgCl 2 , 0.5% BSA, and 2% FBS were incubated with pHrodo red-labeled, heat-killed, serum-opsonized yeast at a MOI of 3 or 10, either at 37°C or at 0°C for 30 min in a volume of 100 μl

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Reducing the accelerating factors of degeneration and combining Chinese and Western medicines in a conservative program through multitarget interventions can not only quickly relieve symptoms but also take into account overall conditioning, which provides a more comprehensive treatment option for patients with early-stage LDD ( Surgery: indication control and principles of selection of surgical procedures Surgery is suitable for patients with ineffective conservative treatment, obvious nerve compression (e.g., decreased muscle strength, urinary and fecal dysfunction) or segmental instability, and the indications need to be strictly grasped