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For cryo-EM analysis, SLC33A1 MAP was solubilized in 2% lauryl maltose neopentyl glycol (Anatrace, NG310), 0.2% cholesteryl hemisuccinate Tris salt (Anatrace, CH210), 50 mM HEPES (pH-adjusted with KOH, pH 7.4), 150 mM KCl supplemented with protease-inhibitor cocktail (1 mM phenylmethylsulfonyl fluoride (PMSF), 2.5 mg ml −1 aprotinin, 2.5 mg ml −1 leupeptin, 1 mg ml −1 pepstatin A) and DNase I

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Vitamin C is involved in collagen synthesis, immune function, and iron absorption