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Store all components at -20°C, and aliquot the biotinylated detection antibody and streptavidin-HRP conjugate into 50μL volumes to avoid repeated freeze-thaw cycles—these steps maintain antibody activity for up to 12 months

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Furthermore, the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, another critical component of the innate immune system, has been implicated in DKD (339)