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Briefly, isolated lysosomes (intact or disrupted) were fixed in 2.5% glutaraldehyde (Polysciences, glutaraldehyde EM grade 25%, 01909-100) in PBS for at least 1 h at room temperature, before centrifugation in a 1.5-ml microcentrifuge tube in an Eppendorf centrifuge 5430R at 14,000 rpm (rotor: FA-45-30-11) for 10 min at room temperature to get a visible pellet

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, Neuroinflammatory response plays a crucial role in the development of neurological diseases