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57,58 Using an azide-containing ncAA to test for incorporation, subsequent dibenzocyclooctyne (DBCO)-biotin treatment and streptavidin pulldown allows for labeling and isolation of AAV particles with tRNA that incorporated the ncAA and separation of mutants that only incorporated natural amino acids into capsid proteins
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Researchers have proposed several mechanisms, including direct effects on gene transcription factors and indirect influences through cellular signaling cascades that regulate telomerase activity
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