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For the differentiation of Th1 cells, the isolated naive CD4 + T cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (Serana, #S-FBS-SA-015, Brandenburg, Germany), 100 U/ml penicillin & 100 µg/ml streptomycin (Thermo Fisher Scientific, #15070063), 10 mM HEPES (Thermo Fisher Scientific, #15630106), 0.05 mM 2-mercaptoethanol (Thermo Fisher Scientific, #21985023), 10 ng/mL IL-12 (Peprotech, #577002), 5 μg/mL anti-IL-4 (Biolegend, #504122)

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[11] [12] ALA may potentiate the effects of insulin and oral hypoglycaemic agents, increasing the risk of hypoglycaemia