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All three isoforms of HK (of low Km) are present in brain tissue, but HK1 is the most abundant isoform in neurons and astrocytes and it is assumed to be “the brain hexokinase.” HKs 1 and 2 bind to the outer mitochondrial membrane by a hydrophobic sequence at their N-terminal, close to the pore that allows ATP output (Pastorino and Hoek, 2008)