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Characterization of a patient-derived variant of GPX4 for precision therapy | Nature Chemical Biology
doi:10.1155/1997/159637
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Generation of HAP1 knockout cell line For human HMBS knockout, we chose a sgRNA targeting an early coding region of the HMBS gene (5’-GCAGCTTGCTCGCATACAGA-3’) and cloned this guide into the pCRISPR_v3.1 plasmid
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