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The immunostaining of cells in culture, cells were fixed for 10 min in 4% paraformaldehyde followed by permeabilization with 0.1% (v/v) triton X-100/PBS for 10 min at RT and blocked with blocking buffer (5% (v/v) goat serum in 0.01% (w/v) triton X-100/PBS) at RT for 1 h

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At 6-months after hospitalization, 90% of patients presented anxiety, depression, and sleep disorders (173), and the most prevalent symptoms reported at 12 months after discharge were anxiety, dyspnea, and fatigue (174)

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