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The mixed samples were then vortexed continuously for 30 min at 4 °C and then centrifuged at 18,000× g for 10 min at 4 °C

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Win-treated GFP cDNA containing plasmid DNA construct has reduced ability to confer green fluorescence phenotype in HEK-293 T cells In an attempt to verify if win-DNA adduct affects gene expression in mammalian cells, we treated pGFP-N1 reporter plasmid with win, purified by ethanol purification and transiently transfected in SV40 T antigen transformed human embryonic kidney HEK-293 T cells