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The culture medium was then collected from the wells and centrifuged, and the secreted level of IL-10 was determined by an ELISA using the commercial Mouse IL-10 Uncoated ELISA Kit (Invitrogen, Thermo Fisher Scientific) ( 2.5 In vitro analysis to evaluate the inhibition of TMA lyase in fecal slurry The fermentation products obtained were tested to evaluate their ability to inhibit TMA production by the microorganisms present in the human fecal slurry

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